Record - 4601

TI- Functional and phenotypic analysis of lymphocytes in head and neck cancer
AU- Snyderman, CH;Heo, DS;Johnson, JT;DAmico, F;Barnes, L;Whiteside, TL
NA- Department of Otolaryngology, University of Pittsburgh (Pa) School of
Medicine, Pittsburgh, PA,
JN- Archives of Otolaryngology - Head and Neck Surgery
PY- 1991
VO- 117
NO- 8
PG- 899-905
DO- Conference
AB- We compared the phenotype and antitumor effector function of lymphocytes
obtained from tumor tissues, lymph nodes, and the peripheral blood of
patients with head and neck cancer. Freshly isolated tumor-infiltrating
lymphocytes were deficient in CD4+ T cells in comparison with lymph node
lymphocytes (LNL) and peripheral blood lymphocytes (LNL) and peripheral
blood lymphocytes. A significantly higher CD4/CD8 ratio observed in LNL vs
tumor- infiltrating lymphocytes and peripheral blood lymphocytes was
attributable to both a significant enrichment in CD4+ T cells as well as a
decrease in CD8+ T cells. The percentage of natural killer cells (CD3 CD56+)
was uniformly low in both tumor-infiltrating lymphocytes and LNL. In
patients with cervical metastases, LNL contained an increased proportion of
CD16- cells. Tumor- involved lymph nodes were not enriched in the CD8+ C11b+
subset of T 'suppressor' lymphocytes compared with uninvolved lymph nodes.
Also, tumor- involved lymph nodes had significantly fewer CD4+ T cells than
did uninvolved lymph nodes. In comparison with peripheral blood lymphocytes,
freshly isolated tumor-infiltrating lymphocytes and LNL were depleted of
cytotoxic effector cells, as indicated by low or absent cytotoxic activity
against tumor cell targets. The ability to generate lymphokine-activated
killer cells was significantly reduced in LNL in comparison with peripheral
blood lymphocytes. In patients with head and neck cancer, depressed local
and regional antitumor responses are associated with a deficiency of
functional cytotoxic effector cells rather than an increase in suppressor T
lymphocytes.
KM- phenotype, lymphocyte, antigen, antibody, head_and_neck_cancer/diagnosis
KN- flow_cytometry, cytotoxicity, assay, lymphokine_activated_killer_cell,
peripheral_lymphocyte, natural_killer_cell, effector_cell, suppressor_cell,
lymph_node, immune_response, squamous_cell_carcinoma, human, male, female,
major_clinical_study, aged, adult

Record - 4602

TI- Can cancer be beaten? (4)
AU- Booyens, J;Smit, BJ
NA- PO Box 59933, Karen Park, South Africa
JN- South African Medical Journal
PY- 1991
VO- 80
NO- 9
PG- 462-463
DO- Letter
KM- cancer_chemotherapy
KN- drug_efficacy, human, aged, child, adult

Record - 4603

TI- Blood transfusions and phenotypic immune profile in head and neck cancer
patients undergoing surgical resection
AU- MacRae, JD;Lampe, HB;Banerjee, D
NA- Department of Otolaryngology, St. Joseph's Health Centre, 268 Grosvenor
Street, London, Ont. N6A 4V2, Canada
JN- Journal of Otolaryngology
PY- 1991
VO- 20
NO- 5
PG- 310-314
AB- Recent papers in the general surgery and otolaryngology literature have
shown a positive correlation between blood transfusion and decreased
survival in cancer patients. This is felt to be due to immunosuppression
(not yet defined) induced by the transfusion. We prospectively analyzed the
peripheral blood immune profiles of patients undergoing surgery for control
of their squamous cell carcinoma of the upper aerodigestive tract. Pre- and
postoperative profiles were examined. T4, T8, natural killer cells, and
interleukin 2 receptor-positive T cell subsets were analyzed by flow
cytometry. Statistical analysis indicated a significant difference between
the non-transfused and transfused subgroups, with the transfused group
having a decrease in their T helper cell and interleukin 2 receptor positive
helper cell populations. This supports other literature which shows an
increased recurrence rate in the transfused patient population. The patients
were analyzed comparing blood transfusions, immune status, initial stage of
disease, the nodal status of the disease, tumor differentiation, and
patient's age yielding transfusion as the greatest single contributing
factor to the observed immunological changes.
KM- immune_deficiency, head_and_neck_cancer/diagnosis,
head_and_neck_cancer/surgery, head_and_neck_cancer/therapy,
blood_transfusion/diagnosis, blood_transfusion/therapy,
head_and_neck_cancer/etiology, blood_transfusion/etiology
KN- squamous_cell_carcinoma, flow_cytometry, human, male, female,
clinical_article, controlled_study, aged, adult